Transgenic Models for Prostate Cancer
Abstract
During phase II of this IDEA Award, our goal was to study genes/pathways implicated in prostate cancer progression using transgenic mouse models. Our efforts focused on creating transgenic models that express 1 of 4 different genes in the mouse prostate. All 4 genes were selected based on studies from our lab and others implicating these genes in human prostate cancer. These include Her-2/neu, cathepsin D, Akt, and Myc. We were successful in generating each of the transgenic mice, and the phenotypes of 3 of them (Cathepsin D, Akt, and Nyc) have been fully characterized. Cathepsin D and Akt mice develop prostatic intraepithelial neoplasia (PIN) only, whereas Myc mice develop PIN that progresses to invasive prostatic adenocarcinoma. In addition, we created transgenic mice expressing the avian retrovirus receptor TVA to permit introduction of multiple transgenes into the prostate in a stepwise manner and to manipulate androgen levels without affecting transgene expression. We documented successful infection with avian retroviruses expressing marker genes (CEP) and are optimizing conditions for transfer of oncogenes. We also generated luciferase transgenic mice, which allow us to visualize the mouse prostate using optimal imaging tools.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2003
- Accession Number
- ADA416618
Entities
People
- Charles Sawyers
Organizations
- University of California, Los Angeles