Rational Derivatives of Maspin as Candidate Tumor Suppressive Agents of Breast Cancer

Abstract

We proposed to generate and characterize rational derivatives of maspin as candidate tumor suppressive agents of breast cancer. We plan to produce these novel maspin variants as purified proteins (Specific Objective 1), and as re-expressed proteins in breast tumor cells that are transfected with the corresponding coding cDNAs (Specific Objective 2). The biological functions of these maspin derivatives as well as the wild type maspin will be examined using comprehensive cellular and biochemical assays that have been established in my laboratory. In particular, we planed to focus on the effect of these molecules on tumor growth, cell adhesion, cell motility and invasion. In addition the biochemical and biophysical properties of these new molecules will be evaluated.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 2002
Accession Number
ADA416651

Entities

People

  • Shijie Sheng

Organizations

  • Wayne State University

Tags

DTIC Thesaurus Topics

  • Albumins
  • Amino Acids
  • Biomedical And Dental Materials
  • Breast Cancer
  • Cell Line
  • Cell Movement
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Culture Media
  • Dna Sequence Analysis
  • Enzyme Inhibitors
  • Epithelial Cells
  • Neoplasms
  • Polymeric Films
  • Proteins

Readers

  • Molecular Biology and Genetics
  • Molecular Genetics

Technology Areas

  • Biotechnology