The Role of Nuclear Beta(II)-Tubulin in Breast Cancer Cells
Abstract
The research is based on our finding that breast cancers contain the Beta II isotype of tubulin inside their nuclei. Our aim is to identify the functional significance of nuclear Beta II. We have used various approaches to this question. We have created another monoclonal antibody, this one specific for the Beta V isotype. We have found Beta V in the microtubules of breast cancer cells, but not in the nuclei. Thus, we have tested the (Beta I, Beta II, Beta III, Beta IVa, Beta IVb and Beta V isotypes in these cells. None is in the nuclei. We have also examined the effect of rhizoxin on these cells; this drug disorganizes the microtubules and causes apoptosis at very low concentrations. Finally, we have used affinity modeling to predict the structures of the various isotypes. We have found that they differ at their taxol-binding sites. This raises the possibility that isotype-specific drugs could be designed that may be more effective than currently used therapies.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2003
- Accession Number
- ADA416661
Entities
People
- Richard F. Luduena
Organizations
- University of Texas Health Science Center at San Antonio