Genome-Wide Nucleic Acid/Protein Interactions in Breast Cancer
Abstract
Since many types of breast cancer remain untreatable, the research proposal aims to develop novel genomic technology to identify potential therapeutic targets and to aid in diagnosing various types of breast cancer at the molecular level. The overarching goal of the proposal is to develop a technology to screen nucleic-acid protein interactions on a genome scale with a focus on understanding complexes involved in breast cancer. In order to identify the regulatory networks of interactions between RNAs and proteins, we propose to develop a rapid genome-scale method to determine the specific RNA targets and RNA binding sites of proteins. The aims were to 1) discover RNA targets of specific RNA binding proteins and protein complexes including the breast cancer susceptibility gene BRCA1, and 2) define the RNA sequences recognized by proteins using novel nanotechnologies including development of optically encoded beads containing both a unique optical signature and a specific oligonucleotide. We have completed the proof of concept experiments for the bead-based assay, identified RNAs that may interact with a BRCA1 complex and are complementing the technology with genome-wide chromatin immunoprecipitation.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2003
- Accession Number
- ADA416691
Entities
People
- Pamela Silver
Organizations
- Dana–Farber Cancer Institute