Roles of Osteonectin in Breast Cancer Metastasis to Bone
Abstract
This research aims to examine differences of cancer-derived osteonectin from vascular-derived and bone cell-derived osteonectin. Of key interest is that these differences may contribute to the affinity of breast cancer cells to bone. During this first year of research, some of the structural differences between the two types of osteonectin have been identified. Breast cancer cells secrete a doublet of osteonectin that is larger than the vascular or bone cell-secreted osteonectin. In addition, breast cancer-secreted osteonectin does not appear to have N-linked glycosyl groups whereas the vascular and bone cell-derived osteonectin is N-linked glycosylated. We have designed three osteonectin affinity columns and have extracted 20-100 mug osteonectin from each cell type. All of these finding support the hypothesis that there are structural differences between breast cancer-secreted and vascular/bone-secreted osteonectin.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2003
- Accession Number
- ADA416714
Entities
People
- Carol V. Gay
- Dianalee A. Campo
Organizations
- Pennsylvania State University