Analysis of the DNA Damage Signaling Network Important For Prevention of Breast Cancer
Abstract
Women with germline mutations in the breast and ovarian cancer gene I (Brcal) have an approximately 50% lifetime of developing ovarian cancer and almost 90% chance of breast cancer. Brcal mutations account for a significant percentage of all breast cancer cases. It appears that the main role for the Brcal protein in cells is to prevent the accumulation of mutations in key growth regulatory genes n response to DNA damage. BRCAL is phosphorylated in response to DNA damage by an elaborate surveillance mechanism called a checkpoint, which detects DNA damage and prevents the accumulation of mutation. We are investigating the role these phosphorylation events play in the regulation of BRCAl. We have mapped phosphorylation sites and will mutate them to determine their function. We are also planning to investigate the mechanism through with the BRCAl protein localizes to sites of DNA damage within cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2003
- Accession Number
- ADA416720
Entities
People
- Stephen Elledge
Organizations
- Baylor College of Medicine