Translational Regulation of PTEN/MMAC1 Expression in Prostate Cancer

Abstract

In this project, we proposed to use a dicistronic expression system to determine whether the long 5'-UTR sequence of PTEN contains internal ribosome entry site (IRES) which can mediate cap-independent translation. In the first year of the study, we have accomplished the proposed work as planed. We found that the long 5'-UTR sequence of PTEN inhibits capdependent translation and that a region in the 5'-UTR sequence of PTEN has an activity to enhance the expression of the second cistron in a dicistronic assay. In addition, we have created a promoterless dicistronic vector and tested it on the 5'-UTR sequence of eIF4G which has been shown to have IRES in previous studies. We found, however, that the IRES activity of eIF4G is a promoter. We plan to test the 5'-UTR of PTEN using the promoterless system and perform a complete analysis of the 5'-UTR of PTEN in the second year of this project.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 2003
Accession Number
ADA416732

Entities

People

  • Jian-Ting Zhang

Organizations

  • Indiana University

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DTIC Thesaurus Topics

  • Amino Acids
  • Biological Factors
  • Biomedical And Dental Materials
  • Carrier Proteins
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Eukaryotes
  • Gene Expression
  • Genetic Code
  • Genetics
  • Growth Factors
  • Neoplasms
  • Organelles
  • Polymeric Films
  • Proteins
  • Transcription Factors

Readers

  • Molecular Genetics
  • Molecular and genetic basis of cancer.