Synthesis of Targeted Drugs for Treating Breast Cancer

Abstract

New chemotherapeutic agents are needed for the improved treatment of breast cancer. In this proposal, we disclose a new approach to the design of anti-cancer drugs. Our method is to synthesize new drug conjugates that incorporate: (1) a specific breast cancer cell -targeting component; (2) a rapid cell membrane translocating/ nuclear localization moiety and; (3) the capability to counter multi-drug resistance mediated by P-glycoprotein and related cellular efflux pumps. The conjugates are prepared in a few synthetic steps from available components. The goal is to demonstrate a proof-of-concept of the effectiveness of this multi-functional drug hypothesis. Specific cancer cell-targeted compounds have been prepared from the breast cancer drug doxorubicin. This cytotoxic agent is covalently linked to a synthetic arginine-glycine- aspartic acid peptidomimetic, which recognizes and binds to a(v)b(3) integrin. This receptor is overexpressed on the surface of breast cancer metastatic cells and tumors. The design also includes incorporation of the Tat peptide analog, H2Narginine(7)COOH, as a rapid cell membrane translocation and effective nuclear localization moiety. The new drugs will be evaluated in breast cancer cell-lines in vitro and in vivo using human breast cancer xenografts in nude mice.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 2003
Accession Number
ADA416752

Entities

People

  • Jerald C. Hinshaw

Organizations

  • University of Utah

Tags

DTIC Thesaurus Topics

  • Aspartic Acid
  • Breast Cancer
  • Cell Line
  • Cell Membrane
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Culture Techniques
  • Drug Resistance
  • Education
  • Energetic Materials
  • Medical Personnel
  • Molecular Biology
  • Neoplasms
  • Organic Chemistry
  • Small Molecules
  • Training

Fields of Study

  • Biology
  • Chemistry

Readers

  • Immunology
  • Molecular Biology and Genetics
  • Prostate Cancer Biology.