Signal Transduction by Designed Metal-Binding Proteins
Abstract
The objective of this research is to develop designed metal-binding proteins to report upon the presence of metal ions in solution. We use small, robust protein frameworks as scaffolds on which to design novel metal-ion binding sites. The metal binding site design is computational, to allow an exhaustive sampling of all possible sites. The sites are designed with defined geometries and a variety of primary ligands to metal, which allow a range of different metal-ion binding specificities and affinities to be arrayed. The aim is to couple the metal-binding event to a change in fluorescence of an appropriate probe. For practical applications, reagent-less systems are preferred, but in the development stages, extrinsic reporter probes are also investigated.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 05, 2003
- Accession Number
- ADA416956
Entities
People
- Lynne Regan
Organizations
- Yale University