TRAIL-Based Radio-Gene Therapy for Prostate Cancer

Abstract

We employed a glucose oxidase (GOD) to deplete glucose in the tumor. TRAIL cytotoxicity is potentiated in the presence of GOD. In vitro and in vivo studies have demonstrated that GOD has potent tumoricidal activity. Hydrogen peroxide produced by GOD is effective in preventing tumor growth, and its effect can be enhanced by hydrogen peroxide decomposition inhibitors such as 3- aminotriazole. Moreover, by comparison to normal cells, tumor cells are more susceptible to GOD. The combination of TRAIL and GOD effectively increased cell death compared to cells treated with TRAIL alone or GOD alone. We further examined the effect of GOD on TRAIL-induced caspase activation. TRAIL-induced activation of caspase was promoted by treatment with GOD. Glucose oxidase enhances TRAIL-induced cytotoxicity. We are currently building on this observation by developing a novel TRAIL-based gene therapy in conjunction with GOD.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 2003
Accession Number
ADA417007

Entities

People

  • Jae J. Song

Organizations

  • University of Pittsburgh

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Blood
  • Breast Cancer
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Free Radicals
  • Gene Expression
  • Gene Therapy
  • Indicator Dyes
  • Mrna
  • Neoplasms
  • Oxidative Stress
  • Prostate
  • Prostate Cancer
  • Proteins
  • Therapy

Fields of Study

  • Medicine

Readers

  • Cellular and Molecular Pathways of Apoptosis.
  • Oncology

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech