Dendritic Cell-Targeted Phage Vectors for Breast Cancer Vaccine Development
Abstract
We hypothesize that one can use specific protein or peptide sequences to direct bacteriophage vectors to dendritic cells. We further propose that one can then use such retargeted phage vectors to deliver potentially important antigens to dendritic cells (DC), and that this may allow one to derive vectors capable of eliciting potent immune responses to breast cancer antigens such as her2. These hypotheses are being experimentally tested. During the period covered by this progress report, we have shown that a novel CD4O-binding peptide (identified by phage display technology) can be used to selectively enhance the transduction of primary DC by mammalian virus vectors (adenovirus, adeno-associated virus). We have also shown that one can insert a mammalian expression cassette encoding GFP into the genome of T7 phage, and that the resulting genomes encode detectable GFP protein in human cells. Finally, we have constructed modified lambda phage coat proteins which are expected to make it possible to selectively direct lambda phage particles to DC.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2003
- Accession Number
- ADA417050
Entities
People
- Stephen Dewhurst
Organizations
- University of Rochester