Discovery of Protein Markers in Breast Cancer by Mass Spectrometry
Abstract
One of the earliest common changes in the development of breast cancer occurs when some breast epithelial cells begin to grow and proliferate independently of estradiol (E2). We have conducted studies on model breast epithelial cell lines: MCF7 cells, whose proliferation is dependent on E2; and LCC1 cells, a cell line derived from MCF7 cells with an acquired E2 independence for growth. We have continued to apply proteomics techniques (two-dimensional electrophoresis, image analysis and protein identification by mass spectrometry) to characterize broadly the patterns of protein expression in these two cell lines and their regulation by E2.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2003
- Accession Number
- ADA417070
Entities
People
- Stephen H. Seeholzer
Organizations
- Fox Chase Cancer Center