Metal Ion Biosensor for Wastewater Discharge
Abstract
A carbonic anhydrase-based metal ion biosensor was optimized for measuring copper in wastewater and seawater using molecular biology methods. CA variants were prepared that enhanced the copper ion specificity and/or decreased the copper affinity were incorporated into the sensor. We have also incorporated two additional, commercially available fluorescent labels (Alexa Fluor 660 and Oregon Green) into the transducer by covalently labeling a CAII mutant containing a single cysteine residue. Finally, we have used made fusions with fluorescent proteins (dsRed-CA) as a new method of transducing metal ion concentration. These methods will be useful in the development of sensors to determine the concentration of metal ions in wastewater and the ocean.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 30, 2003
- Accession Number
- ADA417182
Entities
People
- Carol A. Fierke
Organizations
- University of Michigan