Role of c-myb in Breast Development and Cancer
Abstract
C-MYB, ALONG WITH A-myb and B-myb, belongs to the myb gene family which codes for nuclear proteins that bind DNA in a sequence-specific manner and function as regulators of transcription. There is a large body of evidence to suggest a role for c-myb in breast development and cancer. c-myb is highly expressed in all estrogen receptor positive (ER+) breast tumors as well as ER+ mammary carcinoma cell lines. In addition, our in situ hybridization studies show that c-myb is expressed at high levels in ductal cells from breast tissues of virgin and pregnant mice. To address the role of c-myb in mammary development and cancer, we have created c-myb conditional knockout mice where the expression of this gene is interrupted specifically in the mammary gland using the Cre-lox system. To date, we have generated two female mice that are homozygous for the c-myb floxed alleles (conditional deletion alleles), and additionally, one of the two female mice bears the WAP-cre transgene while the other carries the MMTV-cre transgene. The generation of these breast-specific c-myb conditional knockout mice will afford us the opportunity to dissect the role of c-myb in normal breast development and cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2003
- Accession Number
- ADA417398
Entities
People
- Premkumar E. Reddy
- Yen Lieu
Organizations
- Temple University