Interaction of BRCA1 and p27Kipl Pathway in Breast Cancer

Abstract

Women who have familial breast cancer often have a germline mutation of the breast cancer susceptibility gene known as BRCA1. The function of BRCA1 is not totally understood. Previously, immunochemical analysis of a series of breast cancer cell lines demonstrated a correlation between the expression of p27(kipl)(kipl) and BRCA1. The p27kipl is a member of the universal cyclin-dependent kinase inhibitor family. BRCA1 has a number of activities including DNA repair, growth inhibition, and as a transcription factor. Here we demonstrate that BRCA1 transactivates expression of p27kipl. This transactivation is dependent on - the presence of a functional C-terminal transactivation domain. Promoter- deletion analysis identified the presence of a putative BRCA1-responsive element located at position -615 to -511 of the p27(kipl) promoter.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 2003
Accession Number
ADA417508

Entities

People

  • James L. O'kelly

Organizations

  • Cedars-Sinai Medical Center

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Breast Cancer
  • Cancer
  • Cell Line
  • Cells
  • Gene Expression
  • Inhibition
  • Inhibitors
  • Neoplasms
  • Ovarian Cancer
  • Proteins
  • Regulations
  • Terminals
  • Transcription Factors
  • Tumor Cell Line

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics
  • Molecular and genetic basis of cancer.

Technology Areas

  • Biotechnology