Development of a Novel Tissue Slice Culture Model of Human Prostate Cancer
Abstract
Our goal is to develop a new in vitro model of human prostate cancer. Available models do not faithfully replicate prostate cancer in situ and therefore activities of experimental drugs in these models do not accurately predict clinical efficacy. We are developing "tissue slice culture". Extremely thin slices of fresh tissue are prepared and placed into culture. If conditions are optimal, these slices retain their appropriate structure and functions, providing a realistic model to screen drugs. The tasks were to optimize preparation of slices, develop optimal culture conditions for maintenance, test the validity of these cultures as an accurate in vitro model, and initiate pilot studies to screen candidate therapeutic agents. During this first year, we focused on preparation of slices. This proved challenging due to the unique constituency of prostate tissue, but we obtained tissue cores that we could slice and place into culture. We tested several media and chose one that supported the best maintenance of structure and function. Immunohistochemical protocols were developed to evaluate function of diverse cell types in the tissue. Recently, we tested an experimental compound, vitamin D, for its effects on prostate tissue slices and these slices are ready for evaluation.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2003
- Accession Number
- ADA417612
Entities
People
- Donna M. Peehl
Organizations
- Stanford University