Molecular Basis of Genomic Instability in Breast Cancer: Regulation of the Centrosome Duplication Cycle

Abstract

Alteration in the expression and activity of the centrosomal kinase in breast cancers, Aurora-A/STK15, affect genomic stability, disrupt the fidelity of centrsome duplication, and induce cellular transformation. We followed the proposal tasks (1 and 2) to providence that p160ROCK, aRho-associate Serine/Threonine kinase, associates with STK15 in a protein complex with other SAFs (STK15-associated-factors) and is phosphorylated by STK15 in vivo. Suppression of STK15 by siRNA in Hela cells blocks the ability of centrosomes to organize normal mitotic spindles, induces G2/M arrest and promotes accumulation of tetraploid cells.

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Document Details

Document Type
Technical Report
Publication Date
Jun 01, 2003
Accession Number
ADA417655

Entities

People

  • Gregory Hannon
  • Jianbin Du

Organizations

  • Cold Spring Harbor Laboratory

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Amino Acids
  • Biological Sciences
  • Breast Cancer
  • Cancer
  • Carcinoma
  • Cell Division
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Chromosome Aberrations
  • Cytoskeleton
  • Essential Amino Acids
  • Genetics
  • Genomic Instability
  • Neoplasms
  • Neutral Amino Acids

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics
  • Space Exploration and Orbital Mechanics.