USACEHR Test Report
Abstract
Real-world environmental contamination of native frog species has demonstrated how fragile aquatic ecosystems can be. The South African clawed frog, Xenopus laevis, dwells in water its entire life span and is easily cultured in the laboratory, rendering it a suitable test species for aquatic toxins. Frog embryo growth malformations, and toxicity can easily be assessed with the 96-hour FETAX assay. Drinking water disinfction by-products of different disinfection processes have varying degrees of toxicity. At the request of the Study Sponsor, two bromination by-products and chlorinated surface water (5W) were tested in the FETAX assay with and without the exogenous metabolic activation system (MAS). To further assure reproducibility, the FETAX testing was carried out in two independent laboratories (USACEHR) in Maryland and Oklahoma State University in Oklahoma. The chlorinated surface water was selected by representatives of the American Water Works Association. Surface water was collected from Manatee County, Florida, and shipped to USACEHR. Bromine was added to the surface water prior to chlorination. Tests were performed on free chlorine water and two lots of the same water that had been chlorinated. The test results are contained in this report.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 15, 1999
- Accession Number
- ADA417718
Entities
People
- Henry S. Gardner Jr.
- Margaret W. Toussaint
Organizations
- U.S. Army Center for Environmental Health Research