Designing Rugged Single Molecule Detectors for Stochastic Sensing: A Biological Surface Science Approach

Abstract

This project involved the use of supported phospholipid bilayers to design a new generation of highly rugged single molecule sensors devices. The devices employed alpha-hemolysin, a pore forming protein, as a single molecule transducer element. Our studies demonstrated that this ion channel could be inserted into supported bilayers while retaining two-dimensional mobility. Furthermore, we designed a variety of microfluidic and array-based platforms with concentration and temperature control for displaying this stochastic sensor element.

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Document Details

Document Type
Technical Report
Publication Date
Jun 19, 2003
Accession Number
ADA417745

Entities

People

  • Paul S Cremer

Organizations

  • Texas A&M University

Tags

Communities of Interest

  • Materials and Manufacturing Processes
  • Sensors

DTIC Thesaurus Topics

  • Chemical Compounds
  • Chemical Synthesis
  • Chemistry
  • Crystal Growth
  • Crystals
  • Detectors
  • Lipids
  • Membrane Lipids
  • Molecules
  • Oxygen
  • Phase Transformations
  • Platforms
  • Polymers
  • Proteins
  • Temperature Gradients
  • Transition Temperature
  • Two Dimensional

Fields of Study

  • Physics

Readers

  • Molecular and Cellular Biochemistry
  • Nanoscale Plasmonic Nanotechnology