Evolution of Regulatory Mechanisms in Bacteria
Abstract
Acinetobacter sp. strain ADP 1 is a nutritionally versatile bacterium that exhibits extraordinary competence for natural transformation. This genetic trait greatly facilitates genetic analysis and in the past has been coupled to random PCR-mutagenesis in order to identify how amino acid residues influence function in enzymes and transcriptional activators associated with catabolic pathways. We now have extended this capability to genes from other organisms, in this case Pseudomonas putida, by creating a docking site that allows PCR-amplified P. putida DNA to be integrated into the Acinetobacter chromosome under control of a promoter causing constitutive expression of genes contained in the integrated DNA. Phenotypic expression of the P. putida DNA allows identification of mutants in which the function of the P. putida gene has been altered. This development opens many opportunities for determination of how structure influences function in proteins from a full range of organisms.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 25, 2003
- Accession Number
- ADA417800
Entities
People
- L. N. Ornston
Organizations
- Yale University