Surface Chemistry of Enzymes for Detection and Decontamination of Organophosphorus Compounds
Abstract
The principal objective of this project was to understand the molecular interactions between organophosphorus compounds and the enzymes that catalyze the hydrolysis of these compounds at air water-interface (Langmuir films) and in Langmuir-Blodgett films. The report contains the data on the characterization of the Langmuir films of organophosphorus acid anhydrolase (OPAA), organophosphorus acid hydrolase (OPH), and covalent bonding of the enzyme onto the substrate. Results are encouraging and the studied enzymes form stable monolayers at the interface and can be transferred on to the solid substrate. Film thickness and topography were characterized. We are trying to covalently link the enzyme molecules on to the fiberoptic cable and investigate the fluorescence.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 20, 2003
- Accession Number
- ADA417863
Entities
People
- Roger M. Leblanc
Organizations
- University of Miami