Single-Molecule Detection of Proteins in Supported Bilayers: Applications in Device Fabrication and Sensor Technology

Abstract

Two single molecule fluorescent microscopes were designed and built for exploring membrane protein assembly. The first system, which was designed for use with solid supported membranes, is being used to explore the mechanistic details of alpha-hemolysin assembly. On the other hand, the second system was fabricated for use with blacklipid membranes. It is being employed to correlate ion channel insertion into bilayers with changes in current. The first results with these systems already show great promise.

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Document Details

Document Type
Technical Report
Publication Date
Oct 24, 2003
Accession Number
ADA417870

Entities

People

  • Paul S Cremer

Organizations

  • Texas A&M University

Tags

Communities of Interest

  • Materials and Manufacturing Processes
  • Sensors

DTIC Thesaurus Topics

  • Assembly
  • Chemical Compounds
  • Chemical Synthesis
  • Chemistry
  • Energy Transfer
  • Fabrication
  • Fluorescence
  • Lipids
  • Macromolecules
  • Membrane Lipids
  • Membrane Proteins
  • Membranes
  • Microscopes
  • Molecules
  • Mutant Proteins
  • Optical Equipment
  • Proteins

Fields of Study

  • Chemistry

Readers

  • Molecular and Cellular Biochemistry
  • Nanocomposite Materials Science
  • Software Engineering