Process Research and Development of Antibodies as Countermeasures for C. botulinum
Abstract
This report describes the ongoing project to fill out shell space on the third floor and basement of Othmer Hall, the new home for the University of Nebraska-Lincoln Biological Process Development Facility. At the detailed design is completed and construction is nearly half completed with the expected completion date being December 2003. The third floor will house all of the process research capabilities, i.e. molecular biology, fermentation, cell culture, analytical methods, quality control, purification development and media prep. This report also describes research on the expression of antibodies in Chinese Hamster Ovary (CHO) cells against serotype A botulinum neurotoxin. We have been able to clone and successful express the full intact 8-25 antibody provided by Dr. Jim Marks of UCSF at 30 mgIL in 250 mL spinner shake flasks. This product has been purified and sent to Dr. Leonard Smith of USAMRllD for evaluation. Results are pending.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2003
- Accession Number
- ADA417916
Entities
People
- Michael M. Meagher
Organizations
- University of Nebraska–Lincoln