Adenoviral Gene Therapy Vectors Targeted to Prostate Cancer
Abstract
The overall goal of this project is the development of conditionally-replicating adenoviruses ('CRA') that will selectively infect, replicate in, and kill prostate tumor cells, with the spread of progeny virus to further tumor cells. While CRA have been described before, all have been based on the tropism of the commonly used adenovirus type 5 (Ad5), which 1) does not efficiently infect many tumor cells, and 2) infects a wide variety of normal cells. By improving infection of the tumor cells and the selectivity of conditionally replicating viruses, we hope to improve the therapeutic window associated with replicating Ad treatment. There are three Aims (Tasks) in the project: 1) to evaluate existing Ad serotypes for their possible utility, 2) to use phage display technology to isolate peptides that selectively bind to prostate tumor cells, and 3) to combine the best targeting strategy produced in the first two aims with prostate tumor-selective Ad replication. We have made substantial progress in both of these aims and have identified two very promising fiber proteins for further use. Based on these findings, we feel that we are on track to meet the overall goal of constructing and evaluating a retargeted conditionally replicating virus.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2003
- Accession Number
- ADA417951
Entities
People
- Daniel J. Vonseggern
Organizations
- Scripps Research