Analysis of Breast Cell-Lineage Response Differences to Taxol Using a Novel Co-Culture
Abstract
The purpose of this study is to investigate interactions occurring between normal breast cells and tumor cells grown together in a novel co-culture system. This is accomplished through the use of GFP technology. The scope of the work to date includes establishing optimal growth densities; generating hTERT cell lines to insure the availability of 'normal' cells for continued analysis; and optimizing the conditions for valid efflux and transport experiments. Major findings: Preliminary results suggest that c-myc may be implicated in the ability for the tumor cells to resist Taxol insult through upregulation of the GLUT-1 transport mechanism. In addition, ER negative cells do not appear to be as influenced by the normal cells in co-culture growth experiments. Task associated progress report: We have determined growth curves and patterns for concurrent cell cultures of control epithelial, stromal and tumor cells (Task 1-3) and determined ratios of mammary epithelial, stromal and tumor cell types in co-culture (Tasks 1-3). Concurrently, my Co-PI has established the parameters for valid uptake and efflux transport experiments for normal mammary cells and tumor cells. In order to obtain valid efflux and transport mechanism results for our system, previous methods described in the literature had to be modified.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2003
- Accession Number
- ADA417968
Entities
People
- Lauren S. Gollahon
Organizations
- Texas Tech University