Cloning and Characteristics of Active Egr-1 Target Genes by In Vivo Crosslinking

Abstract

This project is designed toward the identification of a comprehensive set of target genes for the transcription factor Egr1. Since breast cancer cells often do not express Egr1 while normal breast epithelial cells do, it is important to define Egr1 target genes with the hope that critical patterns of Egr1 regulated gene expression active only in normal cells will be revealed. I have chosen to approach this project using an in vivo crosslinking and chromatin immunoprecipitation (ChIp), protocol. Using this approach I have successfully cloned a newly identified Egr1 target gene called TOE1. This gene is currently being characterized, but has the property of an inhibitor of cellular growth when overexpressed. Furthermore, TOE1 may act through interaction and modification of the activity of p53. I have furthered the search for Egr1 target genes using the ChIP approach by adapting an array hybridization protocol using custom generated mammalian gene promoter arrays using a high throughput approach.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 2003
Accession Number
ADA417973

Entities

People

  • Ian De Belle

Organizations

  • Sanford Burnham Prebys Medical Discovery Institute

Tags

DTIC Thesaurus Topics

  • Breast Cancer
  • Cell Physiological Processes
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Chromosome Structures
  • Chromosomes
  • Databases
  • Gene Expression
  • Genetic Code
  • Genetic Structures
  • Identification
  • Neoplasms
  • Peptides
  • Proteins
  • Transcription Factors

Fields of Study

  • Biology

Readers

  • Molecular and genetic basis of cancer.