Transcription-Coupled Repair and Breast Cancer
Abstract
The overall goals of the project were to investigate the consequences of mutations in BRCA1 and BRCA2 in transcription-coupled repair (TCR) of oxidative damage and ultraviolet light induced damage in mouse and human systems. Since others (Gowan et al, 1998) have focused on mouse cell lines, we have focused on human cell lines. We examined the removal of UV light induced cyclobutane pyrimidine dimers (CPDs) from each strand of the DHFR gene in a BRCA1 mutant human cell line HCC1937. We find no evidence for a defect in TCR. We find no evidence for a defect in TCR in a BRCA2 deficient human cell line, Capan-1. Hence, defects in BRCA1 and BRCA2 do not appear to influence transcription-coupled repair of UV damage. The nucleotide excision repair pathway removes UV light-induced damage and bulky adducts formed in DNA by certain carcinogens.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2002
- Accession Number
- ADA417977
Entities
People
- Isabel Mellon
Organizations
- University of Kentucky