Design of Substrates to Study the Interactions of Tumor Cells and Fibroblasts
Abstract
This program was concerned with developing methods that can pattern the attachment of multiple cell types to a common substrate with absolute control over the position, size, shape and identity of each adherent cell. These methods were developed for use in patterning carcinoma cells and stromal fibroblasts in distinct, non-overlapping patterns for mechanistic studies of the inducible expression of stromelysin. Work in the first two years of this program developed the basis for an electroactive surface that could turn on the immobilization of ligands and hence the attachment of cells. Progress in the third period applied the dynamic substrate to pattern the attachment of two different cell types into a coculture array. This important milestone establishes a method to pattern two (or more) cell types to a substrate with arbitrary control over the geometric patterns of each cell type. Work in the fourth, and final, period has developed an electroactive substrate that can electrically release immobilized ligands. These active substrates will permit control over the time in which one patterned cell type is exposed to a second patterned cell type, and hence provide an important methodology for investigating heterotypic cell-cell interactions in cellular culture systems.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2001
- Accession Number
- ADA417979
Entities
People
- Milan Mrksich
Organizations
- University of Chicago