Targeting of Prostate Cancer With Hyaluronan Binding Proteins
Abstract
To test our hypothesis that the hyaluronan (HA) binding proteins (HABP) from cartilage is a new category of anti-tumor agents, we have proposed three aims: (1) To examine the effect of HABP on the tumor growth of prostate cancer cell lines; (2) To examine the effect of link protein and aggrecan on the tumor growth of prostate cancer; (3) To examine the possible anti-angiogenesis effect of HABPs. In past year, we have successfully finished the following works: (1) cloned 1,065 bp of cDNA cording for full length of human cartilage link protein; (2) inserted this cDNA into pcDNA3 expression vector; (3) stablely transfected into tumor cell lines; (4) examined the inhibitory effect of link protein on growth of endothelial cells; (5) demonstrated that the link protein could inhibit the tumor growth in vivo. The significance of these studies are (1) it determines that the link protein is an anti-tumor element in cartilage; (2) the cDNA cloned in this study may be used for gene therapy.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2003
- Accession Number
- ADA417981
Entities
People
- Lurong Zhang
Organizations
- Georgetown University