Genetic and Functional Studies of Genes That Regulate DNA-Damage-Induced Cell Death
Abstract
Studies have shown that apoptosis and survival pathways in response to DNA damage play a critical role in breast cancer development and progression. 90% of breast cancer cases are sporadic where mutations of BRCA1/2 have not been detected. Other breast cancer genes must exist. Our group has approached the issue in two ways, a genomic and a proteomic approach. We have established and utilized a novel retrovirus-based genetic screen system to search for genes that would confer resistance to DNA damage induced apoptosis. Multiple clones have been isolated from this genetic screen. Among the genes identified are both novel and known proteins that may be important in DNA-damage responses. To further elucidate the pathways mediated by BARD 1, we looked for factors interacting with BARD1 using mass spec sequencing. Several factors have emerged from this study and are being examined. The information obtained from our studies should prove useful for developing new and effective screening strategies, drug targets, and treatment for breast cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2003
- Accession Number
- ADA417994
Entities
People
- Zhou Songyang
Organizations
- Baylor College of Medicine