Investigation of Lobular Carcinoma In Situ, Using Molecular Genetic Techniques, for the Involvement of Novel Genes
Abstract
Atypical lobular hyperplasia (ALH) and lobular carcinoma in situ (LCIS), i.e. lobular neoplasia, are lesions of significance in terms of implication of risk to the patient in the development of invasive carcinorna. A strong correlation between the lobular histological type and inactivation of E-cadherin, a protein involved in cell adhesion, has been reported. As well, mutations in the E-cadherin gene have been reported in invasive lobular carcinoma (ILC) and LCIS with adjacent ILC. The purpose of our study is to investigate lobular neoplastic lesions, lacking any adjacent invasive carcinoma, for alterations in and expression of known and novel genes/proteins with the goal of characterizing a molecular genetic profile for lobular neoplasia. We have accrued 23 cases of which there are 14 ALH lesions and 14 LCIS lesions. All cases able to be evaluated are negative for E-cadherin and beta-catenin protein expression by innnunohistochemistry. The mutation analysis has been completed for thirteen lesions and to date alterations in the E-cadherin gene have only been characterized in LCIS. All ALH lesions screened to date show inactivation of E-cadherin but harbor no alterations. Studies are in progress to evaluate loss of heterozygosity at chromosome 16q, E-cadherin promoter methylation, and pl2O-catenin - protein expression. The completion of these analyses will provide insight into the molecular genetic profile for lobular neoplasia.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2003
- Accession Number
- ADA418032
Entities
People
- Irene L. Andrulis
- Teresa L. Mastracci
Organizations
- Mount Sinai Hospital, Toronto