Functional Analysis of the ErbB4 Receptor Tyrosine Kinase
Abstract
ErbB4 is a member of the signaling network composed of the EGF (epidermal growth factor) family of peptide hormones and the ErbB family of receptor tyrosine kinases. We have hypothesized that ErbB4 is a mammary-specific tumor suppressor. Indeed, we have preliminary data that indicates that overexpression of ErbB4 causes the EGF family hormones EGF and Neuregulin to inhibit DNA synthesis of a human mammary tumor cell line. Furthermore, we have constructed three constitutively active, ligand-independent ErbB4 mutants. We have published data indicating that these mutants do not malignantly transform the growth of cultured rodent fibroblasts. These data indicate that ErbB4 is not an oncogene. Moreover, we have preliminary data indicating that one of the three constitutively active ErbB4 mutants inhibits proliferation of several human mammary cell lines. Analyses of additional ErbB4 mutants suggest that this growth inhibition is dependent on ErbB4 kinase activity and on phosphorylation of tyrosine 1056 in the carboxyl-terminal region of the ErbB4 cytoplasmic domain.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2003
- Accession Number
- ADA418035
Entities
People
- David J. Riese
Organizations
- Purdue Research Foundation