Antibody Probes to Estrogen Receptor-Alpha Transcript-Specific Upstream Peptides: Alternate ER-Alpha Promoter Use and Breast Cancer Etiology/Outcome

Abstract

Positive Estrogen Receptor alpha (ER) status correlates with a reduced incidence of breast cancer recurrence in the first years after resection of tumors, and predicts a favorable response to adjuvant anti-estrogens. ER-protein in breast tumors increases with patient age. The project develops novel antibody reagents to probe the expression of ER from alternate promoters taking advantage of short peptides encoded in regions of the ER mRNAs that are promoter specific and not shared. We hope that the short transcript specific peptides will act as Biomarkers' of promoter use. In the second year we have developed a new set of polyclonal antisera to one of the short upstream peptides and these are to be tested in immunohistochemical assays for ability to assess promoter use. A set back has been our failure to develop successful monoclonal antibodies, though the primed mice had good immune responses.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 2003
Accession Number
ADA418096

Entities

People

  • Brian T. Pentecost

Tags

DTIC Thesaurus Topics

  • Antibodies
  • Biomedical And Dental Materials
  • Breast Cancer
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Epithelial Cells
  • Estrogens
  • Gene Expression
  • Genetic Code
  • Genetics
  • Hormones
  • Immune Serums
  • Medical Personnel
  • Neoplasms
  • Tissue Extracts
  • Tumor Cell Line

Fields of Study

  • Biology
  • Medicine

Readers

  • Breast cancer cell signaling and growth regulation.
  • Molecular Genetics
  • Oncology