BRCA2 and Genome Integrity
Abstract
The BRCA2 tumor suppressor gene has been suggested to be important for DNA repair and maintaining genome integrity. Most evidences supporting this hypothesis, however, were obtained from studying mouse embryonic cells. The importance of BRCA2 in maintaining genome integrity in human cells is not very clear. We have completed all tasks of this study. We have generated derivatives of Capan-1 cells, which expressed only mutant BRCA2, that express wild-type BRCA2. Characterization of Capan-1 derivatives showed that expression of wild-type BRCA2 did not have a detectable effect of the sensitivity to gamma-irradiation or DNA damaging drugs of Capan-1 cells. Characterization of MCF12A derivatives showed that reduction of BRCA2 diminished the formation of gamma-irradiation induced RAD51 nuclear foci and increased the sensitivity to gamma-irradiation of MCF12A cells. Unexpectedly, we also found that expression of wild-type BRCA2 suppressed the growth of Capan-1 cells in vitro and in vivo. On the other hand, reduction of BRCA2 appears to inhibit the growth of MCF7 cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2003
- Accession Number
- ADA418196
Entities
People
- Li-kuo Su
Organizations
- The University of Texas MD Anderson Cancer Center