Does the Phenotyping of Disseminated Prostate Cancer Cells in Blood and Bone Marrow Prior to Radical Prostatectomy Provide Prognostic Information?
Abstract
Nearly 20% of men who undergo a radical prostatectomy later relapse with bone metastases. The cellular events that are predictive of subsequent progressive disease remain unknown. We've focused attention on the detection of disseminated prostate cancer (CaP) cells in the blood and bone marrow. Our hypothesis is that these disseminated cells may provide critical insight regarding biomarkers of use in prognostication. We've developed enrichment and isolation techniques that allow the isolation of individual disseminated CaP cells for study as a pool of cells or single cells. Our proposal is to isolate these cells form 50 patients prior to radical prostatectomy and from 10 patients with advanced disease. The cells will be both phenotypically and molecularly analysed. To date 57 patients have been accrued and the analyses well underway. For example, we are finding that 70% of patients prior to radical prostatectomy have disseminated CaP cells in their bone marrow. Also, we show a good correlation between patients with cytokeratin positive cells and human epithelial positive cells. In 36 of 37 specimens having disseminated CaP cells we found aberrant features of chromosome 8 by FISH analysis. Micro-array gene expression analysis is planned on single cells form the first 10 patients to recur.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2003
- Accession Number
- ADA418201
Entities
People
- Robert Vessella
Organizations
- University of Washington