Antibody Microchips to Study Metastasis
Abstract
The hypothesis of my project is that the behavior of an invasive tumor cell is largely determined by a complex collection of numerous proteins on the surface of metastasis cell. I proposed to create a high-density single chain variable fragments (scFvs) antibody chip - that can be employed to compare and quantitate the expression levels of thousands of different plasma membrane proteins on the invasive vs. non-invasive breast carcinoma cell lines. Toward this goal, I have achieved two tasks during this period of award: 1) a - competitive binding condition has been worked out to allow semi-quantification of the amount of antigen bound by individual phage-scFv clone 2) the binding feature of well characterized eight individual phage clones to cell lysate of invasive breast carcinoma MDA-MB-435 cell line provides the initial parameter for the development for a prototype' chip to compare the expression level of a large amount of cell surface proteins by using a 96-well plate format.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2003
- Accession Number
- ADA418683
Entities
People
- Yan Zhang
Organizations
- Sanford Burnham Prebys Medical Discovery Institute