Fibrinolysis in Tumor Associated Angiogenesis
Abstract
Angiogenesis has been shown 0 be necessary for tumor growth and metastasis but specific targets for antiangiogenic therapy in breast cancer have not been identified. Patient samples, a tissue assay of angiogenesis with a mammary vessel explant, and xenograft breast tumors growing in mice will be used to investigate the role(s) of the plasminogen activator/inhibitor/receptor family of proteins in breast cancer angiogenesis. In the second year, we have developed a method for identifying endothelial cells growing in culture of a mammary vessel explant that utilizes Kil-labeled acetylated LDL uptake. This method identifies the endothelial cells with a red fluorescence and%enables observation of living mammary vessel cultures. Construction of retroviral vectors to infect these culture and host mouse tissues with antisense to plasminogen activator/inhibitor/receptor cDNA is underway. Also under construction viral vectors for proteins of interest coupled to green fluorescent protein. Infection of mammary vessel cultures with these viral vectors will yield information on the localization of the laveled proteins and their function in invasion of the matrix and formation of endothelial tubes. Over the next year, we will be using these vectors to study the function of the plasminogen activator/inhibitor/receptor system in the mammary vessel assay and in xenograft tumors in mice.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2003
- Accession Number
- ADA418724
Entities
People
- Sandra W. Mcleskey
Organizations
- University of Baltimore