Analysis of Apaf-1 and Caspase 9 in Tumorigenesis
Abstract
The final goal of our project is to elucidate the apoptotic and cellular senescence mechanisms of tumor suppression in breast cancer. p53 is clearly involved in both apoptosis and senescence, which has led to our emphasis on the p53 pathway . Although the initial approach was a genetical study of the p53-dependent apoptosis pathway, we shifted the focus of study to cellular senescence program, based on our findings of the senescence associated heterochromatic foci (SAHFs). We further characterized SAHFs and found that SAHF-formation is dependent on the pl6/Rb pathway using stable retroviral RNAi system. We also found that E2F-targets, which are required for cell cycle progression and regulated by the Rb-family, are stably silenced in senescence. We propose that E2F-targets are heterochromatinized possibly by being involved in SAHFs, resulting in the irreversible' cell cycle arrest in senescence.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2003
- Accession Number
- ADA418725
Entities
People
- Masashi Narita
- Scott W. Lowe
Organizations
- Cold Spring Harbor Laboratory