The Prostate Expression Database (PEDB)
Abstract
This proposal aims to exploit advances in biotechnology and informatics to develop a genetics resource termed the Prostate Expression Database (PEDB) (http://www.pedb.org). The foundation of PEDB is the identification and characterization of a prostate transcriptome, the intermediary between the genome and the proteome that represents that portion of the human genome actively used or transcribed in the prostate. The research accomplished to date has assembled a working virtual prostate transcriptome that defines the genes used or transcribed in prostate cell types and tissues. This transcriptome has a physical counterpart of >lO,OOO cDNAs arrayed in cDNA microarray format for large-scale expression studies. This transcriptome has been used as a foundation for studies of the prostate proteome, the working counterpart to the genome and transcriptome. Our results show that these approaches are complementary. Analysis of the virtual transcriptome of LNCaP cells has identified 15 new androgen-regulated genes to date. We have extended PEDB to include sequence analysis of the murine prostate and constructed a corresponding database, mPEDB to facilitate the dissemination of mouse prostate gene expression information. The resources generated by the accomplishments of the objectives have utility in identifying genetic alterations in human and mouse prostate carcinoma that can be exploited as diagnostic or therapeutic targets.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2003
- Accession Number
- ADA418729
Entities
People
- Peter S Nelson