Novel Lishmania and Malaria Potassium Channels: Candidate Therapeutic Targets
Abstract
The purpose of this research project is to characterize the newly identified potassium channel genes from Plasmodium falciparum and determine if they may be exploited as chemotherapeutic targets. The research plan, included: complete cloning of two P. falciparum K+ channels; subcloning of channel genes for expression and epitope tagging; expression of the channel genes for biophysical, pharmacological and biochemical analyses; generation of specific antibodies to channel proteins for analysis of channels in vivo; and pharmacological analysis of specific K+ channel blockers as anti-malaria agents. In the first year of this project we have made considerable progress in reaching these goals. We have completed the cloning and subcloning of both K+ channel genes from P. falciparum, PFKl and PFK2. We have expressed booth genes and identified protein products in bacterial and mammalian heterologous expression systems. We have generated specific antibodies using recombinant proteins as antigens. These antibodies recognize the appropriate proteins by Western blot analysis. We have screened a panel of K+ channel blockers and have consistent data on their anti-malarial activity. We are encouraged by the progress this year and are confident of further success and reaching our stated goals as we continue with this project.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2003
- Accession Number
- ADA418746
Entities
People
- Kami Kim
- Thomas J. McDonald
Organizations
- Albert Einstein College of Medicine