Marine Mammal Health: Development of Immunodiagnostic and Viral Diagnostic Methodologies and Reagents

Abstract

Reagents and methods/procedures were developed to permit evaluation of general humoral immune status and immune status relative to specific pathogens in cetaceans marine mammals. Immunoglobulin isotypes IgG1, IgG2, IgA, and IgM were purified and poly- or monoclonal antibodies specific for these isotypes were developed. ELISA assays for - immunodiagnosis of past exposure to Erysipelothrix rhusiopathiae, a bacterial pathogen of cetaceans, were developed. Tissue culture lines from three species of cetaceans were established and evaluated for virus susceptibility and culture characteristics.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Nov 29, 2003
Accession Number
ADA418900

Entities

People

  • B. L. Middlebrooks
  • R. A. Patterson

Organizations

  • University of Southern Mississippi

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Animals
  • Antibodies
  • Antigens
  • Biology
  • Cell Line
  • Cells
  • Cetaceans
  • Immune Serums
  • Immunoglobulins
  • Mammals
  • Marine Mammals
  • Microbiomes
  • Molecules
  • Pathogenic Bacteria
  • Proteins
  • Tissue Culture
  • Viruses

Fields of Study

  • Biology

Readers

  • Immunology
  • Virology (or Medical Virology).