The Role of a FGF-Binding Protein in Breast Cancer
Abstract
We studied the expression and regulation of the secreted FGF-BP protein that can enhance FGF activity and studied Its impact in vivo. In human tissues we found that FGF-BP is significantly elevated in its expression in invasive breast cancers (approximately 112 of invasive breast cancers) and rarely found expressed in normal breast tissue or in non-invasive in situ carcinoma. We report a significant, positive correlation of FGF-BP expression with the estrogen receptor status of the cancers. We demonstrate transcription factors binding to the FGF-BP gene promoter and show that members of the C/EBPbeta family are crucial transcriptional factors binding to the promoter. We show regulation by EGF of this binding. We initiated the generation of conditional, regulated transgene expression in mice due to the embryonically lethal phenotype observed with constitutively active promoters driving the transgene. As an alternative model, transient transgene expression in chicken embryos is initiated to assess to what extent embryonic toxicity can be monitored in this new model.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2002
- Accession Number
- ADA418908
Entities
People
- Anton Wellstein
Organizations
- Georgetown University