The Role of a FGF-Binding Protein in Breast Cancer

Abstract

We studied the role and regulation of a secreted, FGF binding protein (FGF-BP) in breast cancer. We established a method to detect FGF-BP mRNA in paraffin-bedded archival breast cancer samples using in situ hybridization. We show feasibility of the technique for breast cancer tissues. We surveyed breast cancer cell lines and show that EGF-receptor overexpressing MDA-MB 468 human breast cancer cells express high levels of FGF-BP mRNA that is detectable by Northern blot analysis. We demonstrate that the FGF-BP gene is regulated in these breast cancer cells by the growth factor EGF. We show that the MAP kinase p38 is crucial in the transduction of the EGF signal towards FGF-BP regulation in MDA-MB 468 cells.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2000
Accession Number
ADA418912

Entities

People

  • Anton Wellstein

Organizations

  • Georgetown University

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Biomedical And Dental Materials
  • Blood
  • Carcinoma
  • Carrier Proteins
  • Cell Line
  • Cells
  • Cellular Structures
  • Chemistry
  • Fibroblasts
  • Growth Factors
  • Membranes
  • Molecules
  • Neoplasms
  • Polymeric Films
  • Polysaccharides
  • Proteins

Fields of Study

  • Biology

Readers

  • Aerospace Engineering
  • Breast cancer cell signaling and growth regulation.