Dynamic Tissue Culture from Prostate Biopsy Specimens as a model for Predicting Tumor Radiosensitivity to Ionizing Radiation Treatment
Abstract
Prostate cancer is the most common non-cutaneous malignancy in men. Radiation therapy is a common treatment for this disease however, most patients receive a similar dose of radiation (70-76 Gy) regarless of individual clinical, pathological, or molecular characteristics of the tumor. The hypothesis of this project is that all prostate carcinomas are unique and that by identifying specific tumor markers or other molecular characteristics using our dynamic tissue culture system (Parrish et al, 2002), we can identify those tumors most sensitive to radiation therapy. The specific aims for the first year were to use prostate biopsy tissue, obtained retrospectively, and adapt our organ culture technique to the requirements of prostate biopsy specimens. We have been able to determine the optimal biopsy core size and tissue culture medial conditions. We have also demonstrated that basal cells present in the prostate glandular tissue proliferated over the 72 hour time period of organ culture. We have maximized the length of time that tissue remains viable in our dynamic tissue culture system. We are now ready to begin Aim II of the proposal determining the baseline readiosensitivity of prostate tissue and assessing the roles of p53, bcl-2, and NFKB in the intrinsic radiosensitivity of prostate tissue. We hope to further profile these biomarkers and using them to predict prostate tissue radiosensitivity will aid in the diagnosis and prognosis of this significant cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2003
- Accession Number
- ADA418949
Entities
People
- David W. Nyman
Organizations
- University of Arizona