Alpha Synuclein in a Model of Multiple System Atrophy

Abstract

Multiple system atrophy (MSA) is a progressive neurological disorder characterized by parkinsonism, cerebellar dysfunction and autonomic impairment. The cardinal pathological feature of MSA is the glial cytoplasmic inclusion (OCI) in oligodendrocytes, and a major component of OCIs is a-synuclein. We identified an oligodendrocytic precursor cell line, CG-4, that endogenously produces a-synuclein. We characterized alpha synuclein-immunoreactive (alpha synuclein-IR) material in the CO-4 cells (with affection to higher molecular weight forms), and studied the effects of oxidative stress. We also produced CO-4 cells that constitutively overexpress human alpha-synuclein and CO-4 cells that express human alpha-synuclein under control of the tetracycline-ON (inducible promoter) system, and we have characterized both of these.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2003
Accession Number
ADA419316

Entities

People

  • Clifford W. Shults

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Astrocytes
  • Biomedical Research
  • Cell Line
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Diseases And Disorders
  • Infection
  • Materials
  • Medical Personnel
  • Neurosciences
  • Organic Chemistry
  • Oxidative Stress
  • Precursors
  • Stem Cells
  • Two Dimensional

Fields of Study

  • Biology

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