The Effect of Recombinant Factor VIIa and Fibrinogen on Bleeding from Grade V Liver Injuries in Coagulopathic Swine
Abstract
This was a 2 part study. The first part of the study was performed to determine if recombinant factor VIIa would reduce bleeding after a grade V liver injury in hypothermic, dilutionally coagulopathic pigs when used as an adjunct to abdominal packing and to determine the optimal dose of the drug. The second part of the study was performed to compare different fluids for resuscitation of this model. Fluids were compared for their effects on volume of resuscitation, rebleeding, metabolic effects and their effects on coagulation. The grade V liver injury model is well described and a clamp is utilized creating a reproducible injury. In part 1 of the study animals underwent a 60% of blood volume isovolemic exchange transfusion with 5% human albumin and the animals' temperature was maintained at 33 degrees C. In part 2, animals were normothermic and non-coagulopathic. The study revealed that rFVIIa reduces blood loss form the grade V injury by approximately 50%. This effect was not changed by increasing the dose form 180 micrograms/kilogram to 720 micrograms/kilogram. In the second part of the study, we showed that resuscitation with normal saline requires 3 times as much fluid and results in severe acidosis and coagulopathy compared to resuscitation with lactated Ringers solution.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2003
- Accession Number
- ADA419372
Entities
People
- Martin A. Schreiber
- Susan I. Brundage
Organizations
- Baylor College of Medicine