Reversal of Doxorubicin Resistance in Human Breast Adenocarcinoma (MCF-7) Cells by Liposomal Monansin

Abstract

We have previously shown that stealth monensin liposomes (SML prepared by pH-gradient method) enhances the cytotoxicity of doxorubicin by a factor of 16.5 in MCF-7/dox cells. In the present study, we investigated the effect of SML on the induction of apoptosis and its influence on the expression of multidrug resistance, MDR-1 and multidrug resistance associated protein, MRP-1 in doxorubicin resistant human breast adenocarcinom (MCF-7/dox) cells. Apoptosis was studied by TUNEL staining and the expression of multidrug resistance genes (MDR-1 and MRP-1) was elucidated by RT-PCR.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 2003
Accession Number
ADA419392

Entities

People

  • Mandip

Organizations

  • Florida A&M University

Tags

DTIC Thesaurus Topics

  • Adenocarcinoma
  • Apoptosis
  • Biological Staining And Labeling
  • Biomedical Research
  • Breast Cancer
  • Cancer
  • Cell Line
  • Cells
  • Drug Resistance
  • Indicator Dyes
  • Inhibitors
  • Molecular Biology
  • Neoplasms
  • Particle Size
  • Resistance
  • Tumor Cell Line

Fields of Study

  • Biology
  • Medicine

Readers

  • Oncology (Cancer Research).