Behavior and Characterization of Prostatic Stem Cells
Abstract
The first aim identifies prostatic stem cells b a novel in vivo approach using our normal prostatic basal and luminal cell lines. The second aim studies the characteristics of prostatic tumors arising after transformation of these cells. We established a novel intraprostatic transplantation assay in which GFP-tagged basal cells engraft and differentiate into luminal cells. The basal cells also differentiate into luminal cells when inoculated under the renal capsule (RC). This indicates that these cells have stem cell features as they self-renew and give rise to mature luminal progeny in two in vivo assays. We also show that transformed basal and luminal cells form subcutaneous tumors and that the rate of tumor growth is enhanced by normal prostatic smooth muscle (SM) cells. This indicates that SM may contribute to the progression of prostatic tumors and that paracreine signaling between epithelial and SM cells may promote carcinogenesis. Intraprostatic or sub-RC inoculation of transformed basal cells gives rise to tumors that contain luminal cells. This indicates for the first time that tumorigenic basal cells (arising form transformed stem cells) can give rise to prostatic tumors with a luminal phenotype. This is relevant as it explains how prostate cancer could originate in basal stem cells yet manifest clinically as a tumor expressing luminal features.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2003
- Accession Number
- ADA419488
Entities
People
- Elaine L. Wilson
Organizations
- New York University