Electron Transfer of Redox Enzymes to Inorganic Electrodes

Abstract

This project was to develop a protein and peptide-based nanowire and nanoswitch with defined length and properties for a broad spectrum of application in nanobiotechnology. These nanowires were used to develop novel enzyme/conductive matrix in which the activity of redox enzymes could be controlled by direct electron transfer to inorganic electrodes. We investigated synthetic peptide structures as linking agents between target enzymes and conductive electrodes. Directly couple nanocrystals to amino acids, peptide and proteins. This was carried through several methods. One was to couple the nanocrystals to peptides and proteins directly. But the yield was very low and become prohibitively expensive. The alternative was to first couple the nanocrystals to amino acids and through chemical synthesis to make peptides and proteins. We have successfully achieved it.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2003
Accession Number
ADA419613

Entities

People

  • Shuguang Zhang

Organizations

  • Massachusetts Institute of Technology

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Amino Acids
  • Chemical Compounds
  • Chemical Engineering
  • Chemical Synthesis
  • Chemistry
  • Covalent Bonds
  • Electrodes
  • Electron Transfer
  • Electrons
  • Immobilized Enzymes
  • Materials
  • Nanocrystals
  • Nanomaterials
  • Nanotechnology
  • Nanowires
  • Physical Chemistry
  • Scientific Research

Fields of Study

  • Chemistry

Readers

  • Molecular and Cellular Biochemistry
  • Nanocomposite Materials Science

Technology Areas

  • Biotechnology
  • Microelectronics