Derivation of Double-Targeted Adenovirus Vectors for Gene Therapy of Prostate Cancer
Abstract
The subject of the present studies is the development of adenovirus (Ad)-based vector capable of selectively infecting and killing prostate cancer cells. The intended use of such a vector is for gene therapy of prostate cancer patients, whereby the virus administered to patients would find, infect and destroy tumor cells. Development of this vector is to employ the concepts of conditionally replicative Ad (CRAd) and genetic targeting of Ad to tumor-specific cell surface molecules (transductional targeting). Conditional control over the replication of the vector is to be achieved by using the previously established osteocalcin (OC) promoter. Targeting of the virus to prostate tumor cells will be accomplished via genetic incorporation into its capsid of peptide ligands, which selectively bind to a major marker of prostate tumors, prostate-specific membrane antigen (PSMA). Identification of these targeting peptides by phage library biopanning is the central task of the project. The efficiency of the resultant Ad therapeutic is to be tested by using the virus for the treatment of prostate tumor xenografts established in immunodeficient mice.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2004
- Accession Number
- ADA419820
Entities
People
- Victor Krasnykh
Organizations
- University of Alabama